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Popular Features. New Releases. Description This is an up-to-date atlas of the stereotaxic coordinates of the beagle brain. It provides stellar illustrations of the organization of nerve tracts and the morphology of the nuclei that compose the central nervous system. Product details Format Spiral bound pages Dimensions x x 7. No one will need photocopies. This digital edition began in as a scan of a library copy. At the time, we were getting into neurotoxicology and needed neuroanatomy references to locate specific brain regions, nuclei, and fiber tracts. Like others, we were unable to purchase a canine neuroanatomy text so we settled for a borrowed library copy of Dr.
Luckily, we had an over-sized scanner available as the pages of the original text are The sections reproduced in the book were obtained from the brains of three disease-free beagle dogs of the same litter, aged five months, raised at the Virus Research Laboratories of Cornell University. Each brain was sectioned in one of the three planes: horizontal, sagittal, and transverse.
The dogs were anesthetized by intraperitoneal injection of Nembutal solution and then were perfused through the heart with a physiological saline solution followed by a solution of 10 per cent formalin. The brains then were removed, immersed in a solution of the same strength of formalin for a few weeks, dehydrated in graded alcohols, and slowly infiltrated with celloidin. The embedded brains were cut at 35 microns and the sections numbered consecutively. Selected sections were stained by the iron-hematoxylin method of Loyez for myelin sheaths after mordanting in alum of iron Bertrand.
Adjacent sections, which alternated with those stained for fibers, were stained for nerve cell bodies according to the cresyl violet method of Bielschowsky-Plien Bertrand. Loyez sections are more useful for studying overall detail than sections stained by the Nissl method, and the present atlas is confined to sections stained for fibers. Indeed, nuclei, for example, of the thalamus often can be distinguished more clearly in such sections. However, constant reference has been made to sections of the cresyl violet series in locating nuclear groupings in Loyez sections.
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During dehydration and embedding, the brains shrank to about three-fourths to two-thirds of the size of the fixed brain. The sections were numbered consecutively: the sagittal sections, from left to right; the horizontal sections, from ventral to dorsal; and the transverse sections, from rostral to caudal. The orientation of the sections corresponded closely to the desired plane. However, the sagittal sections were tilted about 1 mm from the true plane so that the midline section lies approximately in section number plate 59 for the medulla but in about section for the rostral part of the brain stem.
The transverse plane corresponded to a cut from the posterior commissure to the caudal extremity of the mamillary bodies see transverse section , plate Since the brain stem of the dog, unlike that of the human, is flexed only slightly at the mesencephalon, reorientation of the embedded specimen was not required, and the sections of the upper and lower brain stem deviated only slightly in direction from one another.
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Buy securely. Book of the Month. Authors Tim Winton Sarah J. Top Pick. This is an up-to-date atlas of the stereotaxic coordinates of the beagle brain.
It provides stellar illustrations of the organization of nerve tracts and the morphology of the nuclei that compose the central nervous system. For immediate download. Check your local Dymocks store for stock.